| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.244269 |
| Chromosome: | chromosome 6 |
| Location: | 3163108 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g275450 | (1 of 1) PF00069//PF13855 - Protein kinase domain (Pkinase) // Leucine rich repeat (LRR_8) | 3'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGTGCGCGCGGCACCCAACTCTTTCTCTG |
| Internal bar code: | AGGCACTCGCGTAAGATGACAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 581 |
| LEAP-Seq percent confirming: | 96.2298 |
| LEAP-Seq n confirming: | 1072 |
| LEAP-Seq n nonconfirming: | 42 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTGGGACGTACTGGGAGTG |
| Suggested primer 2: | CAACAGCCTCACAGTCTCCA |