Insertion junction: LMJ.RY0402.244272_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.244272
Chromosome:chromosome 12
Location:7673144
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre12.g554250 LPB1 (1 of 1) PTHR11952:SF8 - UDP-GLUCOSE PYROPHOSPHORYLASE 3; Low Photochemical Bleaching protein 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGTGTCTTGCGTGATTGCGTGGTACGGGAG
Internal bar code:CGAACCGACATCAGTCGACCGA

Confirmation - LEAP-Seq

LEAP-Seq distance:580
LEAP-Seq percent confirming:99.0329
LEAP-Seq n confirming:512
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:CTACAATGACGCAGGGGATT
Suggested primer 2:TGCTTCTTTTCAGCATCACG