Insertion junction: LMJ.RY0402.244279_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.244279
Chromosome:chromosome 6
Location:1612782
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre06.g260700 XUV1,UAPA6 (1 of 2) K06901 - putative MFS transporter, AGZA family, xanthine/uracil permease (pbuG); Xanthine/uracil/vitamin C permease-like intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGTGCATAGCCGTGGTACACCCACAAAGAT
Internal bar code:CTAGGTGAAGTCATGGACGGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:587
LEAP-Seq percent confirming:99.2208
LEAP-Seq n confirming:764
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:17

Suggested primers for confirmation by PCR

Suggested primer 1:CAAGTCCACAGCAACGCTTA
Suggested primer 2:ATTGAGGTGTGTGCGTGTGT