| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.244315 |
| Chromosome: | chromosome 9 |
| Location: | 1286320 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g399200 | SDR19 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCAGCGAGGGAGGCATGTAGTAGAGGCGG |
| Internal bar code: | GGCGTAATCCGGATACGAACCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 751 |
| LEAP-Seq percent confirming: | 78.4339 |
| LEAP-Seq n confirming: | 4237 |
| LEAP-Seq n nonconfirming: | 1165 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTCAACTTCCAAAAGAGCG |
| Suggested primer 2: | TAGCACGATGCTCGCAATAC |