| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.244318 |
| Chromosome: | chromosome 6 |
| Location: | 1080997 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g256650 | (1 of 4) PTHR10887//PTHR10887:SF331 - DNA2/NAM7 HELICASE FAMILY // HELICASE MOV10L1-RELATED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTTCACTGCTAGGCACAGGGCAGCTGGCC |
| Internal bar code: | TGACGTTGAGCACCGGACTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 491 |
| LEAP-Seq percent confirming: | 98.9071 |
| LEAP-Seq n confirming: | 181 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCGTACCGTCATGTCTTCT |
| Suggested primer 2: | AACTTCGTAGTTGGCATGGG |