| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.244338 |
| Chromosome: | chromosome 2 |
| Location: | 4685643 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g101600 | (1 of 3) PF11735 - Cryptococcal mannosyltransferase 1 (CAP59_mtransfer) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGTAGGGCCGCTTGCCGTCCCAGCGGTGC |
| Internal bar code: | ATAAGTTCTTGGTCATGGTTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 67 |
| LEAP-Seq percent confirming: | 91.6667 |
| LEAP-Seq n confirming: | 11 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGGCAAGAAGGATGTGGTG |
| Suggested primer 2: | GATTCCTCCTCCTCCTCACC |