Insertion junction: LMJ.RY0402.244347_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.244347
Chromosome:chromosome 6
Location:7893908
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre06.g303200 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACTACTGTTGTTGTTGTTGTTGTTGTCGTT
Internal bar code:CATGTGAAATAAAGGGGTGGGC

Confirmation - LEAP-Seq

LEAP-Seq distance:636
LEAP-Seq percent confirming:99.51
LEAP-Seq n confirming:4265
LEAP-Seq n nonconfirming:21
LEAP-Seq n unique pos:55

Suggested primers for confirmation by PCR

Suggested primer 1:ACCTGGTGCATGAACGTGTA
Suggested primer 2:GAGCGAGAACAACGAACACA