| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.244403 |
| Chromosome: | chromosome 8 |
| Location: | 3730499 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g377100 | ADK6 | Adenylate kinase; (1 of 1) PTHR23359//PTHR23359:SF94 - NUCLEOTIDE KINASE // ADENYLATE KINASE 1, MITOCHONDRIAL-RELATED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTTTAGGCAACAAGTGGTTGGGAGCAGGG |
| Internal bar code: | CAGCGGACGGGATCTCAGGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 505 |
| LEAP-Seq percent confirming: | 99.458 |
| LEAP-Seq n confirming: | 1835 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGAAGGGGTTCATCCTCGAC |
| Suggested primer 2: | TTTTAAATGGTCAACCCCCA |