Insertion junction: LMJ.RY0402.244404_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.244404
Chromosome:chromosome 13
Location:1627704
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre13.g573550 ANK26 Predicted protein with ankyrin repeats; (1 of 7) PF12796//PF13637 - Ankyrin repeats (3 copies) (Ank_2) // Ankyrin repeats (many copies) (Ank_4) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTTCACTACCTTCCCGTCCCTCTCATGTG
Internal bar code:GGTTCTCCATGCTCGGTCACTA

Confirmation - LEAP-Seq

LEAP-Seq distance:542
LEAP-Seq percent confirming:98.8439
LEAP-Seq n confirming:171
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:ACCTTTCTTTGGGCCTGG
Suggested primer 2:TCCGTCAACACAGTGGACAT