| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.244456 |
| Chromosome: | chromosome 13 |
| Location: | 1882016 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g575701 | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGGCTTCCGCACCTTCAGCATGTCTCCAC |
| Internal bar code: | CGATCACGTCGGCCCCGGTGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 485 |
| LEAP-Seq percent confirming: | 95.7071 |
| LEAP-Seq n confirming: | 379 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCAGGAGCAGGAGTGACAT |
| Suggested primer 2: | ACCAGGAGACACACCTGTCC |