| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.244490 |
| Chromosome: | chromosome 16 |
| Location: | 5179101 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g683200 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACGTGGAGGCGCCGGCAGACCGCAGTGCG |
| Internal bar code: | GTGTGTCGGATTATGTCCCGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 597 |
| LEAP-Seq percent confirming: | 99.173 |
| LEAP-Seq n confirming: | 1559 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 46 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAACCCGTCCGAACTTTTTA |
| Suggested primer 2: | CCATACATGCCTATTCCGCT |