| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.244502 |
| Chromosome: | chromosome 7 |
| Location: | 2500649 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g329550 | VFL5 | Variable flagella protein 5 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAAGGCAATGCTCAAGGAGCAGTAGCCCGT |
| Internal bar code: | CGTCTCCTAGATGCAGTTGCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 622 |
| LEAP-Seq percent confirming: | 96.4912 |
| LEAP-Seq n confirming: | 55 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGAAGCCAGCGCTATTTGAA |
| Suggested primer 2: | AGCACCTACTGTACCCGCAG |