| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.244553 |
| Chromosome: | scaffold 50 |
| Location: | 11410 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre50.g761497 | (1 of 1) PTHR13890:SF0 - MAGNESIUM TRANSPORTER MRS2 HOMOLOG, MITOCHONDRIAL | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCTCGAGTTCTACCTGCAGCGGGCCTCC |
| Internal bar code: | ATGCTTGGAATTCAGAAGCATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 705 |
| LEAP-Seq percent confirming: | 99.531 |
| LEAP-Seq n confirming: | 2122 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGCTTTCGATATGGTCTGT |
| Suggested primer 2: | ACATGAGGTAAGGCCGTGAG |