Insertion junction: LMJ.RY0402.244606_3

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.244606
Chromosome:chromosome 13
Location:3741596
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre13.g589450 FBB6L1 (1 of 1) IPR002035//IPR013694 - von Willebrand factor, type A // VIT domain; FBB6 like protein 1 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGAAGCAGCGGCGTAGACGGTCGCTGAGAC
Internal bar code:GGGGAACTGGCGACGCTCGGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:586
LEAP-Seq percent confirming:96.6312
LEAP-Seq n confirming:545
LEAP-Seq n nonconfirming:19
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:GAGGTAGCTGAACTCCACCG
Suggested primer 2:AACATGCCTGGTTGTAAGCC