| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.244628 |
| Chromosome: | chromosome_8 |
| Location: | 3900249 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre08.g378200 | PPO1 | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TTAGCGGCGCCCTGCGCCGTCGACCCTGGG |
| Internal bar code: | GTGGCTAAAAGGATCTACTTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 545 |
| LEAP-Seq percent confirming: | 99.6988 |
| LEAP-Seq n confirming: | 14895 |
| LEAP-Seq n nonconfirming: | 45 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACCCTAGACACGACAGCAA |
| Suggested primer 2: | GCCGTCTCCCTATGCAATAA |