| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.244634 |
| Chromosome: | scaffold 19 |
| Location: | 36010 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre19.g750397 | (1 of 1) 3.2.1.17 - Lysozyme / Muramidase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTGGCCGTGCCCTGTTGTCTACCGCCGT |
| Internal bar code: | CGAAGAAGAGGGTCTGTGCGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 542 |
| LEAP-Seq percent confirming: | 89.0921 |
| LEAP-Seq n confirming: | 1364 |
| LEAP-Seq n nonconfirming: | 167 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACGAGTGCTACAACCTCCA |
| Suggested primer 2: | ATGTTGTAAGGTTGGAGGCG |