| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.244638 |
| Chromosome: | chromosome_1 |
| Location: | 2649573 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre01.g015500 | CGL91 | sense | 5'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GTTTTTGTCAGCTTCGGGCCGCTTCGTCAC |
| Internal bar code: | GTCGGAGGAAGGTATGCAGAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 553 |
| LEAP-Seq percent confirming: | 98.5857 |
| LEAP-Seq n confirming: | 1185 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTTGCAACGCTGTAAGCAG |
| Suggested primer 2: | AAGACAGGGTGGAAGGTGTG |