Insertion junction: LMJ.RY0402.244666_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.244666
Chromosome:chromosome 5
Location:3392964
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre05.g241050 (1 of 228) IPR029063 - S-adenosyl-L-methionine-dependent methyltransferase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GATACTTCCTACGTTGGTCAATGACAAACA
Internal bar code:GAACCAATAATGGAAAGGAATC

Confirmation - LEAP-Seq

LEAP-Seq distance:355
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:56
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:TCCAATTCACAGACGAGCAG
Suggested primer 2:GGACCCGGTTCATAGCAGTA