| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.244675 |
| Chromosome: | chromosome 3 |
| Location: | 8949286 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g208833 | (1 of 1) K10896 - fanconi anemia group M protein (FANCM) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTCTTCGTCAGACCGGAACTCCACACGCG |
| Internal bar code: | GTCGCAGCCGGCTCGGTGGTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 394 |
| LEAP-Seq percent confirming: | 88.0383 |
| LEAP-Seq n confirming: | 184 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACGTGCGTCTTCCAATACC |
| Suggested primer 2: | ACTGCCCATATACAGCGGAC |