| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.244677 |
| Chromosome: | chromosome 16 |
| Location: | 279975 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g694200 | MMP15 | Matrix-metalloproteinase-like protein; (1 of 40) 3.4.24.38 - Gametolysin / Lysin | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATGCAATGCCCGCAGCGCATGGTTGGTGG |
| Internal bar code: | CTCATTGTCGTCGTGCATGGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 338 |
| LEAP-Seq percent confirming: | 99.6681 |
| LEAP-Seq n confirming: | 3003 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCTAGCGATAGCCAGTCC |
| Suggested primer 2: | TCACTCCCCTACCCACTCAC |