Insertion junction: LMJ.RY0402.244688_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.244688
Chromosome:chromosome 14
Location:388220
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre14.g610566 (1 of 3) PF02209 - Villin headpiece domain (VHP) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACTGGGCTTCTGGCCGGTGGAGCTTCCAGA
Internal bar code:CCGGGGACCGGCGATTGGTCCT

Confirmation - LEAP-Seq

LEAP-Seq distance:572
LEAP-Seq percent confirming:99.5338
LEAP-Seq n confirming:427
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:TGTAGCGGCAGCAGTGATAC
Suggested primer 2:ATCATTGCCCTCACAACTCC