| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.244688 |
| Chromosome: | chromosome 14 |
| Location: | 388241 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g610566 | (1 of 3) PF02209 - Villin headpiece domain (VHP) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAACCTGTTCTCGGCCCCCCCCCCTTCCCC |
| Internal bar code: | CATTCAGTACGTCGCCGGACTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 254 |
| LEAP-Seq percent confirming: | 89.8734 |
| LEAP-Seq n confirming: | 284 |
| LEAP-Seq n nonconfirming: | 32 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTAGCGGCAGCAGTGATAC |
| Suggested primer 2: | ATCATTGCCCTCACAACTCC |