| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.244691 |
| Chromosome: | chromosome 2 |
| Location: | 984528 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g080200 | TRK1 | Transketolase, chloroplastic; (1 of 1) 2.2.1.1 - Transketolase / Glycoaldehyde transferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTATGATAGAGGAGTCGGTGTATCCGCTGA |
| Internal bar code: | GTAGGTAGGTGCAAATTTGTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1015 |
| LEAP-Seq percent confirming: | 99.6802 |
| LEAP-Seq n confirming: | 9351 |
| LEAP-Seq n nonconfirming: | 30 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACCAACGTAATGGGAGCAG |
| Suggested primer 2: | CTACACCATCCACGACACCA |