| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.244703 |
| Chromosome: | chromosome 9 |
| Location: | 6230831 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g406200 | PRORS1,TSO1 | organellar class II (G, H%252C P and S) tRNA synthetase; (1 of 1) PTHR11451//PTHR11451:SF6 - TRNA SYNTHETASE-RELATED // SUBFAMILY NOT NAMED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTGAAGGCAGGGGTGCGTTGGGGCTTTT |
| Internal bar code: | GTCATTCTCCCAAGCAACCGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1201 |
| LEAP-Seq percent confirming: | 98.7039 |
| LEAP-Seq n confirming: | 6016 |
| LEAP-Seq n nonconfirming: | 79 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCTAGGTGGGACATGCTAA |
| Suggested primer 2: | ACAATGTCGTAAACACCGCA |