| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.244735 |
| Chromosome: | chromosome 16 |
| Location: | 6767737 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g673841 | (1 of 1) PTHR13481:SF0 - UPF0454 PROTEIN C12ORF49 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGGCATTCAGCGGCCTGGCGACCGACCTG |
| Internal bar code: | GCCTGTACTAATAGGCGTATCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 426 |
| LEAP-Seq percent confirming: | 95.7143 |
| LEAP-Seq n confirming: | 134 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCTAGCAAGGTTTCCTGCG |
| Suggested primer 2: | AGAAGTCCATCATGGGCAAG |