Insertion junction: LMJ.RY0402.244771_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.244771
Chromosome:chromosome 11
Location:2946015
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre11.g478128 GCN20 Soluble ABC-F domain-containing protein related to GCN20; (1 of 1) K06158 - ATP-binding cassette, subfamily F, member 3 (ABCF3) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCTTCACGCTCCCCATACCCACACACACC
Internal bar code:CGTTGCTATTAGAAAAACAGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:366
LEAP-Seq percent confirming:46.8531
LEAP-Seq n confirming:804
LEAP-Seq n nonconfirming:912
LEAP-Seq n unique pos:31

Suggested primers for confirmation by PCR

Suggested primer 1:AGGAGAAGACAGGGGAGGAG
Suggested primer 2:TCTTCCGTGCCTAGCTCACT