Insertion junction: LMJ.RY0402.244816_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.244816
Chromosome:chromosome 14
Location:3300061
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre14.g629840 (1 of 22) PTHR11017//PTHR11017:SF145 - LEUCINE-RICH REPEAT-CONTAINING PROTEIN // SUBFAMILY NOT NAMED 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCCCTAGATCCTACTGACTCTTCTAATTCT
Internal bar code:GTCGCTGCCTCCCGAGGACGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:515
LEAP-Seq percent confirming:99.798
LEAP-Seq n confirming:494
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:15

Suggested primers for confirmation by PCR

Suggested primer 1:AAAGTAAGTTTCCCGGCGTT
Suggested primer 2:GAATGCAAACGCTGTGAAGA