| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.244822 |
| Chromosome: | chromosome 1 |
| Location: | 4178771 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g027650 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTGCCCTGGCGCGTGAGGGCCTCTTCCTC |
| Internal bar code: | AAGTCTAGGCGGGTGTTGCGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 587 |
| LEAP-Seq percent confirming: | 92.7326 |
| LEAP-Seq n confirming: | 638 |
| LEAP-Seq n nonconfirming: | 50 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAACGCTGCTACACCCTGTG |
| Suggested primer 2: | TTATGGATGTCGAGGACGTG |