| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.244825 |
| Chromosome: | chromosome 9 |
| Location: | 76228 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g386250 | SMM33 | S-adenosyl-L-methionine-dependent methyltransferase; (1 of 228) IPR029063 - S-adenosyl-L-methionine-dependent methyltransferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTATTGCAGTGCCCTCCATGGGTGCGACA |
| Internal bar code: | GCCCGGCGACCCCGACTAGGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 541 |
| LEAP-Seq percent confirming: | 99.1653 |
| LEAP-Seq n confirming: | 1188 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATGGCGTCTTATTGTCGGT |
| Suggested primer 2: | GGACGGATTGATTGACAGGT |