Insertion junction: LMJ.RY0402.244833_3

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.244833
Chromosome:scaffold 19
Location:72719
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre19.g750547 NDA2 thylakoid NADH dehydrogenase; (1 of 3) K17871 - NADH:ubiquinone reductase (non-electrogenic) (ndh1) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCGACCCCAACCATATAACCCGCGTCCTT
Internal bar code:CGGGAAGCGATGTGATGGGCCT

Confirmation - LEAP-Seq

LEAP-Seq distance:349
LEAP-Seq percent confirming:97.7099
LEAP-Seq n confirming:128
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:ATACACACACGCCTTCCCTC
Suggested primer 2:AACAACACGTTTGGCATCAA