| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.244847 |
| Chromosome: | chromosome 1 |
| Location: | 6275195 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g044850 | (1 of 1) K17592 - sacsin (SACS) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGCGACGTCCCGGCTCATTCGCGAGCATG |
| Internal bar code: | TAGTGAAATTGAGACATAATCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 158 |
| LEAP-Seq percent confirming: | 92.9577 |
| LEAP-Seq n confirming: | 66 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTTGCAGGTTGACGTGTTC |
| Suggested primer 2: | CAAATGCTCAAAGTGCCAGA |