| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.244873 |
| Chromosome: | chromosome 17 |
| Location: | 5963919 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g740350 | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCTCCTGCTGAGTATGCCACGCTACATTT |
| Internal bar code: | CTTTGGCTACAGCGAACGGTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 413 |
| LEAP-Seq percent confirming: | 99.7972 |
| LEAP-Seq n confirming: | 492 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCGCCTCAGAGATACTGGC |
| Suggested primer 2: | AAGTTGGTCCAAAGTCCACG |