| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.244880 |
| Chromosome: | chromosome 2 |
| Location: | 6742605 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g118350 | MFT14 | Major facilitator superfamily transporter; (1 of 82) IPR020846 - Major facilitator superfamily domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGAGGGTATGGACAGCGCCGCGGCGCAAT |
| Internal bar code: | GAGCGGACCTAGGGGTCGCGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 98 |
| LEAP-Seq percent confirming: | 99.0909 |
| LEAP-Seq n confirming: | 109 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGCTGTAGATGAGTGCTGG |
| Suggested primer 2: | AGGCATGTGCGTTTCCTACT |