Insertion junction: LMJ.RY0402.244902_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.244902
Chromosome:chromosome 2
Location:1928334
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre02.g087850 PTK19 Protein tyrosine kinase; (1 of 2) 2.7.10.2//2.7.11.25 - Non-specific protein-tyrosine kinase / Cytoplasmic protein tyrosine kinase // Mitogen-activated protein kinase kinase kinase / MLTK CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCCACCACCGCTGCTGCCGGCGCACGTGG
Internal bar code:TATTGTCTGTCCAGGATAATAG

Confirmation - LEAP-Seq

LEAP-Seq distance:373
LEAP-Seq percent confirming:99.7197
LEAP-Seq n confirming:2846
LEAP-Seq n nonconfirming:8
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:CCACACACACACACGTCGTA
Suggested primer 2:CCCACAACAAACTCACGATG