| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.244945 |
| Chromosome: | chromosome_11 |
| Location: | 3322111 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre11.g480250 | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CGATGTAACAGCACCATGACCACACCCACC |
| Internal bar code: | GGGCCAATGGGCAGTTATTTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 269 |
| LEAP-Seq percent confirming: | 99.2076 |
| LEAP-Seq n confirming: | 2629 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTTGAGAAGCCTGGACAAG |
| Suggested primer 2: | ACAGGTAGATGGGTGCCTTG |