| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.244946 |
| Chromosome: | chromosome 10 |
| Location: | 4238772 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g450200 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAAAGTAAAAGCACGTGCATAATGCTCTCT |
| Internal bar code: | GGACAGGTTTCTTCTTATCATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1223 |
| LEAP-Seq percent confirming: | 96.2302 |
| LEAP-Seq n confirming: | 485 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTGTGTTGGCCTTGAGGTT |
| Suggested primer 2: | CTGTCCCATCACACATCCAG |