| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.244947 |
| Chromosome: | chromosome_12 |
| Location: | 2595427 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre12.g505150 | OPR52 | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TGGCGCGTTCACCGGGCCACCTGTGATGCG |
| Internal bar code: | TCTTCTGTCACTTAACGAGTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 491 |
| LEAP-Seq percent confirming: | 99.3951 |
| LEAP-Seq n confirming: | 18238 |
| LEAP-Seq n nonconfirming: | 111 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAGTTGGATTGTGGCAAGT |
| Suggested primer 2: | AACGTTCTCAAGACGTGGCT |