Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.244997 |
Chromosome: | chromosome_10 |
Location: | 4250898 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre10.g450350 | IFT25,FAP232 | Intraflagellar transport protein 25 | sense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TGGTCTGGCTTGTCCTGGTCGCACTTTTCT |
Internal bar code: | GTGGATCAACGACGAGCGCGAA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 362 |
LEAP-Seq percent confirming: | 99.6171 |
LEAP-Seq n confirming: | 7024 |
LEAP-Seq n nonconfirming: | 27 |
LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TTCCTTTCCGCTCTGGTCTA |
Suggested primer 2: | TTAAGGCATTGCTGGGTAGC |