| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.245014 |
| Chromosome: | chromosome 3 |
| Location: | 7568712 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g204800 | RTA2 | (1 of 2) 3.6.3.16 - Arsenite-transporting ATPase / Arsenite-translocating ATPase; Arsenite-transporting ATPase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACACACACACGCATCTGTAACGCCAATGG |
| Internal bar code: | GTGAGGCTGCGAGTGCAAGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 544 |
| LEAP-Seq percent confirming: | 98.8722 |
| LEAP-Seq n confirming: | 263 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCTCATGTCGTGTGTGGAG |
| Suggested primer 2: | AGCGTTGGGGTATCATGAAG |