| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.245022 |
| Chromosome: | chromosome 11 |
| Location: | 2899470 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g477900 | protein of unknown function | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGTGTTGTTGTTGACGTGTGCTGTGGGAG |
| Internal bar code: | GTAGGCTGTTGCACTCGCAGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 208 |
| LEAP-Seq percent confirming: | 99.5583 |
| LEAP-Seq n confirming: | 2705 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGCATGTTCTTTGAGGAGG |
| Suggested primer 2: | CTCACCGTGTCAAAACCCTT |