| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.245023 |
| Chromosome: | chromosome 12 |
| Location: | 8343770 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g549450 | (1 of 1) 3.7.1.5 - Acylpyruvate hydrolase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCGCTGCAATGGGCCCCGTGCCGCCGATC |
| Internal bar code: | TCCACTTACCCCTTAAGAATGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 636 |
| LEAP-Seq percent confirming: | 99.4698 |
| LEAP-Seq n confirming: | 1501 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 33 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCCAAGCTCAAGTCCAAGC |
| Suggested primer 2: | GGTCTAGAAGACGCCGACAG |