| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.245035 |
| Chromosome: | chromosome 6 |
| Location: | 1914627 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g263400 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTCATTCGCTCCCCCGTCCCTTCGTCCAG |
| Internal bar code: | ACGACGGAAACTTCTATCACAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1079 |
| LEAP-Seq percent confirming: | 99.4523 |
| LEAP-Seq n confirming: | 9624 |
| LEAP-Seq n nonconfirming: | 53 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAGACCTGCAAGGTAGGCT |
| Suggested primer 2: | TCAGGGAATAAAGACGCCAC |