| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.245044 |
| Chromosome: | chromosome 1 |
| Location: | 2553189 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g014800 | MSH7,CPL21 | putative MutS type 2 DNA repair protein; (1 of 1) K07456 - DNA mismatch repair protein MutS2 (mutS2) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCGTGATGACTTTGCAGGACTCACGTTAC |
| Internal bar code: | CATGTTGTTGTTTTTATCAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 678 |
| LEAP-Seq percent confirming: | 99.5662 |
| LEAP-Seq n confirming: | 459 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTTCACCGTCACCATGTCA |
| Suggested primer 2: | CCTGTGCCTCCTCTTTTGAG |