| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.245062 |
| Chromosome: | chromosome 2 |
| Location: | 62140 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g073500 | FAP241 | Flagellar Associated Protein 241 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGAGTATGAGCTGTTGCGCTAGGGGATCC |
| Internal bar code: | CGGGCGGAGGATGGACAAGTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 613 |
| LEAP-Seq percent confirming: | 98.1559 |
| LEAP-Seq n confirming: | 1650 |
| LEAP-Seq n nonconfirming: | 31 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCACACCTGCTCTTTAAGC |
| Suggested primer 2: | AACTTTTAGGCCCGACGATT |