| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.245087 |
| Chromosome: | chromosome 14 |
| Location: | 1792821 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g619900 | PHX22,PFH22 | Putative prolyl 4-hydroxylase; (1 of 1) PTHR10869:SF80 - IRON ION BINDING / OXIDOREDUCTASE | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACCCATACCACCCATACCACCCACCCCAC |
| Internal bar code: | GAGCCATAAAGCGCGTCCTAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 376 |
| LEAP-Seq percent confirming: | 87.1612 |
| LEAP-Seq n confirming: | 611 |
| LEAP-Seq n nonconfirming: | 90 |
| LEAP-Seq n unique pos: | 41 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCAACAAACACCCCTTTT |
| Suggested primer 2: | GTGTGTGTGTGCGTGTGTGT |