| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.245088 |
| Chromosome: | chromosome_12 |
| Location: | 8704645 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre12.g546100 | KIN14-2,FAP125 | Kinesin-like motor protein, kinesin-14 (C-terminal motor) family | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GAGTTGGGAGCGATGACCACAGATTTGGAC |
| Internal bar code: | TCCGGTTGGTACCCAGTCGTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 265 |
| LEAP-Seq percent confirming: | 99.7736 |
| LEAP-Seq n confirming: | 3967 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCACCATCATACCATCACCA |
| Suggested primer 2: | CTATATCCGCGGCAATGAAT |