Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.245106 |
Chromosome: | chromosome 9 |
Location: | 6805495 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
intergenic |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTTGAATGCATGTGACCGTACAACAAGCA |
Internal bar code: | GTCGCACCCTTTCCCGACCCTA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1071 |
LEAP-Seq percent confirming: | 98.4709 |
LEAP-Seq n confirming: | 19641 |
LEAP-Seq n nonconfirming: | 305 |
LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ACACAAATGCCATAACGCAA |
Suggested primer 2: | CAAGTAAGAGGTTCGCTGCC |