| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.245115 |
| Chromosome: | chromosome 10 |
| Location: | 814281 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g423350 | (1 of 1) PF07052 - Hepatocellular carcinoma-associated antigen 59 (Hep_59) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTCCTTTGTTTACCAGACATGTCTCAGGT |
| Internal bar code: | CGTCGCGCCGTCTGAGTGAGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 117 |
| LEAP-Seq percent confirming: | 99.1632 |
| LEAP-Seq n confirming: | 237 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTGTGTGGTGTGTTGATGA |
| Suggested primer 2: | TACAACGCGTATGGGAATGA |