| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.245120 |
| Chromosome: | chromosome 5 |
| Location: | 324788 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g233300 | (1 of 1) 2.1.1.98 - Diphthine synthase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCTTTCATTCCCCTGCACGGCTACACACA |
| Internal bar code: | GGAGAACGTCAGGAGCATGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 247 |
| LEAP-Seq percent confirming: | 13.4021 |
| LEAP-Seq n confirming: | 247 |
| LEAP-Seq n nonconfirming: | 1596 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTTCTTGACGCACACAGGA |
| Suggested primer 2: | CATACGCATACACACTGCCC |