| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.245126 |
| Chromosome: | chromosome 9 |
| Location: | 2114786 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g393650 | (1 of 1) PF00069//PF01590 - Protein kinase domain (Pkinase) // GAF domain (GAF) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGAAATAAAAATATGTATGAAAGAAAAATG |
| Internal bar code: | GAATATGCTTCTATAAGCATGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 733 |
| LEAP-Seq percent confirming: | 98.2473 |
| LEAP-Seq n confirming: | 5998 |
| LEAP-Seq n nonconfirming: | 107 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCCCCTTACGCTCATTCAC |
| Suggested primer 2: | CTGGTCCTGTGGGGAGAATA |